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Dissolve, warming a little bit. Sterilize within an autoclave employing a validated cycle, at a temperature not exceeding one hundred fifteenRefrigerate the suspension if It's not at all made use of inside 2 hrs.] Figure out the volume of cfu for every mL in Just about every suspension, using the situations of media and microbial recovery incubatio
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A seed-stock procedure really should be useful for extended-term storage of cultures. Cultures gained within the ATCC needs to be resuscitated Based on Instructions. If grown in broth, the cells are pelleted by centrifugation. Resuspend in 1/twentieth the volume of fresh new maintenance broth, and add an equal volume of 20% (v/v in water) sterile g